A fascinating study has found that sniffing female tears significantly reduced male aggression and decreased activity in aggression-related brain networks. It’s suggested that the effect, which is caused by chemical signals in tears and is also seen in rodents, serves a protective function.
But that makes it more complex because you have to start worrying about the order they’re done in because it might be different emotions playing your first or third game plus the effect might linger, take time to show, etc.
Far better to answer one simple question and prove there is an effect then follow up tests can look at finding the bounds to that and starting to narrow in on identifying mechanics.